Engineers find imaging technique could become treatment for deep vein thrombosis

Date:
July 15, 2021
Source:
Penn State
Summary:
Researchers set out to develop technology capable of localizing
and imaging blood clots in deep veins. Turns out their work may not
only identify blood clots, but it may also be able to treat them.



FULL STORY ==========================================================================
Penn State College of Engineering researchers set out to develop
technology capable of localizing and imaging blood clots in deep
veins. Turns out their work may not only identify blood clots, but it
may also be able to treat them.


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The team, led by Scott Medina, assistant professor of biomedical
engineering, published its results in Advance Healthcare Materials.

"Deep vein thrombosis is the formation of blood clots in deep veins,
typically in a person's legs," said Medina. "It's a life-threatening
blood clotting condition that, if left unaddressed, can cause deadly
pulmonary embolisms - - when the clot travels to the lungs and blocks an artery. To manage DVT, and prevent these life-threating complications,
it's critical to be able to rapidly detect, monitor and treat it."
The challenge, according to Medina, is that current diagnostic imaging
methods lack the resolution required to precisely pinpoint potential
breeding grounds for clots and monitor the clots in real time. DVT can sometimes present as swelling and aching in a person's leg, which can
then be examined via ultrasound.

"Ultrasound isn't great for diagnosing DVT," Medina said. "It can tell
you that a region of fluid flow may look odd, which might be related to a
clot -- but maybe not. You follow up with blood tests to look for specific factors, and, together, you might be able to diagnose a clot." Once a
clot is diagnosed, a clinician may order either pharmaceuticals to help
break it apart or a procedure that involves snaking a probe to the clot
to grab it and physically remove it from the body. The pharmaceuticals
may not be enough to break the clot apart, though, or they could trigger bleeding issues elsewhere in the body, while the procedure option is
invasive and carries risks, including potential infection.



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To better identify the location, composition and size of clots, which
informs how to treat them, Medina and his team used a particle approach
they developed in 2017. Called nanopeptisomes (NPeps), the particles
comprise a shell around a droplet of fluorine-based oil similar to liquid Teflon. The surface of the shell holds a molecule that finds and binds a protein on the surface of activated platelets, a key cellular component
of clots.

"The particles bind to the surface of the clots, we apply the ultrasound,
and the droplet turns to gas and forms a bubble under the shell," said
Medina. "It gives an excellent contrast for imaging. The bubbles appear
exactly where the clots are forming." But, Medina said, a mystery
unfolded as they tested their technique. To analyze how to diagnose
and treat clots, the researchers first induce clots in bovine veins by injecting an enzyme that triggers clot formation.

"The enzyme induces clot formation generally 100% of the time -- but
when we applied the particles, we only saw clot formation about 30%
of the time," Medina said. "We had to wonder: were the particles not
only binding to the clots, but somehow breaking them down?" The team
tested its hypothesis, but the researchers would lose the bubble signal
after 15 minutes of ultrasound every time.

"We think that once our particles start to decorate the clot, they
saturate the surface and inhibit the mechanisms of further clot growth,"
said Medina. "And under the ultrasound, the particles are disrupting the
clot or inhibiting its mechanism to persist. While we don't understand
the underlying mechanism yet, it's clear that these particles can image
and help treat clots in real time." The researchers plan to continue investigating how the particles are disrupting the clots, as well as
develop more control over how the particles behave.

The National Science Foundation Faculty Early Career Development Program,
a Penn State Multidisciplinary Seed Grant and a Penn State Graduate
Research Fellowship supported this work in part.

========================================================================== Story Source: Materials provided by Penn_State. Original written by
Ashley J. WennersHerron.

Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Janna N. Sloand, Eric Rokni, Connor T. Watson, Michael A. Miller,
Keefe
B. Manning, Julianna C. Simon, Scott H. Medina.

Ultrasound‐Responsive Nanopeptisomes Enable Synchronous
Spatial Imaging and Inhibition of Clot Growth in Deep Vein
Thrombosis. Advanced Healthcare Materials, 2021; 2100520 DOI:
10.1002/adhm.202100520 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2021/07/210715124532.htm

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